show Abstracthide AbstractWe used RNA-seq to profile gene expression changes during flg22 activated pattern-triggered immunity in multiple Brassicaceae including Capsella rubella, Cardamine hirsuta and Eutrema salsugineum as well as in multiple Arabidopsis thaliana accessions. This allows comparative transcriptomics within and across species to investigate the evolution of stress-responsive transcrption changes in these species. Overall design: Gene expression profiling via mRNA sequencing of 12-day-old seedlings treated with mock or 1µM flg22 for 1 h, 9 h or 24 h. We used the A. thaliana accessions Can-0, Col-0, Gy-0, Kn-0, Kon and No-0 and the Brassicaceae species Capsella rubella, Cardamine hirsuta and Eutrema salsugineum