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SRX4198450: GSM3187644: Donor 1 MCTL; Homo sapiens; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 59.1M spots, 2.9G bases, 1.2Gb downloads

Submitted by: NCBI (GEO)
Study: Human antimicrobial cytotoxic T lymphocytes, defined by NK receptors and antimicrobial proteins, kill intracellular bacteria
PRJNA475765 • SRP150351 • All experiments • All runs
show Abstracthide Abstract
Human cytotoxic T cell subsets were sorted based on cytotoxic molecule expression and RNA sequencing was performed on these subsets Overall design: Human T cells from two donors were sorted by Flow based on expresssion of Granzyme B, Perforin and Granulysin in to subsets of cells experssing non of these markers, expressing ony granzyme B (monocytotoxic - MCTL) expressing granzyme B and perforin (dicytotoxic -DCTL), expressing all three (Tri-cytotoxic, TCTL - also called Polycytotoxic PCTL). RNA sequencing was performed on these sorted subsets and gene expression was compared to generate specific signatures of these subsets allowing for surface marker identification that permitted isolation for functional study.
Sample: Donor 1 MCTL
SAMN09401608 • SRS3409024 • All experiments • All runs
Organism: Homo sapiens
Library:
Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: RNA was isolated according to the MARIS protocol (Hrvatin, S et al. MARIS: method for analyzing RNA following intracellular sorting. 2014 PLoS One Vol 9, e89459) RNA libraries were prepared for sequencing using standard Illumina protocols
Experiment attributes:
GEO Accession: GSM3187644
Links:
Runs: 1 run, 59.1M spots, 2.9G bases, 1.2Gb
Run# of Spots# of BasesSizePublished
SRR729585459,104,5242.9G1.2Gb2018-08-31

ID:
5685394

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