GSM3168344: L2C4: Spinal cord; Rattus norvegicus; RNA-Seq - SRA

SRX4167876: GSM3168344: L2C4: Spinal cord; Rattus norvegicus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 1500) run: 2.4M spots, 86.5M bases, 108.9Mb downloads

Submitted by: NCBI (GEO)

Study: Genome wide analysis of the lower lumbar spinal cord at 4 days after T9 hemisection injury

PRJNA474209 • SRP149801 • All experiments • All runs

show Abstracthide Abstract

The goal of this study is to elucidate the influence of hemisection injury at thoracic spinal cord (T9) on the transcriptome of the lower lumbar spinal cord at acute phase. mRNA profiles of spinal cord at 4 days-post injury and before injury were generated. 72 Differentially Expressed Genes (DEGs) were observed. Our study represents the detailed analysis of transcriptomes of spinal cord distal to the hemisected lesion at acute phase, with biologic replicates, generated by RNA-seq technology. Overall design: The effect of spinal cord injury on the transcriptome of intact lower spinal cord was investigated.

Sample: L2C4: Spinal cord

SAMN09288675 • SRS3361229 • All experiments • All runs

Organism: Rattus norvegicus

Library:

Instrument: Illumina HiSeq 1500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Spinal cords were removed, flash frozen in liquid nitrogen, and RNA was harvested. Library were constructed in accordance with the CEL-Seq2 protocol and prepared on the Illumina HiSeq1500 platform.

Experiment attributes:

GEO Accession: GSM3168344

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 2.4M spots, 86.5M bases, 108.9Mb

Run	# of Spots	# of Bases	Size	Published
SRR7263740	2,402,840	86.5M	108.9Mb	2024-05-21

ID:: 5654557

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