U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

SRX4119626: GSM3151848: Jej_3_L; Homo sapiens; RNA-Seq
1 ILLUMINA (Illumina HiSeq 4000) run: 8.7M spots, 434.5M bases, 144.9Mb downloads

Submitted by: NCBI (GEO)
Study: Human enteroendocrine cell transcriptomic profiling
show Abstracthide Abstract
We purified two populations of human jejunal enteroendocrine cells (GLP1+ and GLP1-) by FACS and identified transcripts enriched in endocrine cell lineages. Overall design: Comparative transcriptomic study of 3x cell populations by bulk RNAseq, with 11 biological replicates
Sample: Jej_3_L
SAMN09245816 • SRS3334440 • All experiments • All runs
Organism: Homo sapiens
Library:
Instrument: Illumina HiSeq 4000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Human jejunal mucosal samples were dissociated to single cells, fixed in paraformaldehyde (4%), permeabilised with Saponin, Antibody labelled against GLP-1, CHGA and SCG2 and FACS sorted into 3x populations (GLP1-/CHGA+/SCG2+ [Single positive]; GLP1+/CHGA+/SCG2+ [Double positive]; triple negative [Neg]). RNA was extracted using the Ambion Recoverall total Nucelic Acid isolation kit following the MARIS protocol and concentrated using a Qiagen RNeasy Minelute cleanup kit. The library was constructed with 4ng of input RNA using the Clontech Smarter Stranded total RNA-seq - Pico Input mammalian kit v1
Experiment attributes:
GEO Accession: GSM3151848
Links:
Runs: 1 run, 8.7M spots, 434.5M bases, 144.9Mb
Run# of Spots# of BasesSizePublished
SRR72124538,690,818434.5M144.9Mb2019-01-01

ID:
5605187

Supplemental Content

Search details

See more...

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...