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SRX3985525: GSM3107955: LC14_Ctrl_starved; Caenorhabditis elegans; RNA-Seq
1 ILLUMINA (Illumina HiSeq 4000) run: 38.5M spots, 2G bases, 637.1Mb downloads

Submitted by: NCBI (GEO)
Study: mRNA of fed and starved C. elegans L1 larvae 3 generations following control, short-term dauer or long-term dauer conditions
show Abstracthide Abstract
N2 worms were subjected to control (well-fed), short-term dauer (6 d), or long-term dauer (40 - 49 d) conditions. Worms were recovered and maintained well-fed for 3 generations. F3 progeny were collected as fed or starved L1 larvae. Overall design: 6 conditions include: control fed, control starved, short-term dauer fed, short-term dauer starved, long-term dauer fed, and long-term dauer starve. 3-12 biological replicates were sequenced per condition
Sample: LC14_Ctrl_starved
SAMN08969136 • SRS3210299 • All experiments • All runs
Library:
Instrument: Illumina HiSeq 4000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Total RNA was extracted using TRIzol with minor modifications to the manufacturer's protocol. 1mL of TRIzol was used per sample. Samples were homogenized using 100uL of acid-washed sand. Libraries were prepared using the NEB Next Ultra Non-directional mRNA-seq Kit.
Experiment attributes:
GEO Accession: GSM3107955
Links:
Runs: 1 run, 38.5M spots, 2G bases, 637.1Mb
Run# of Spots# of BasesSizePublished
SRR705449038,491,3482G637.1Mb2018-07-27

ID:
5453356

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