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SRX392688: GSM1290020: ChIP-4b_wild-input; Homo sapiens; ChIP-Seq
1 ILLUMINA (Illumina Genome Analyzer IIx) run: 24.2M spots, 2.4G bases, 1.1Gb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: Genome-wide screening for ELF4-binding sites.
show Abstracthide Abstract
ELF4 (also known as MEF) is a member of the ETS family of transcriptional factors. While an oncogenic role has been demonstrated for ELF4 mainly in hematopoietic malignancies, its definitive function in human carcinogenesis is not clear yet. Here we demonstrate that a wide array of human tumors carry somatic, loss-of-function mutations in ELF4 for its transcriptional activity, and restoring its function exerts anti-proliferative effects. To further explore the tumor suppressive function of ELF4, its binding sites were searched among the human genome with the use of chromatin immunoprecipitation coupled with sequencing (ChIP-seq). Overall design: Examination of binding site for FLAG-immunoprecipitated mock, ELF4 (WT) and ELF4 (L211M) with each input sample as a control.
Sample: ChIP-4b_wild-input
SAMN02444804 • SRS516205 • All experiments • All runs
Organism: Homo sapiens
Library:
Instrument: Illumina Genome Analyzer IIx
Strategy: ChIP-Seq
Source: GENOMIC
Selection: ChIP
Layout: PAIRED
Construction protocol: T3M-1 Cl-10 cells were infected with a mock retrovirus or virus generated from pMXS-ires-EGFP expressing FLAG-epitope–tagged ELF4 or ELF4(L211M). ChIP was conducted with anti-FLAG M2 antibody (Sigma-Aldrich). The precipitated DNA fragments were subjected to massively parallel sequencing with Genome Analyzer IIx (Illumina) for 51 bases from both ends of the fragments. ChIP-seq libraries were prepared using Illumina NEBNext ChIP-Seq Library Prep Reagent Set following the manufacturer's protocols.
Experiment attributes:
GEO Accession: GSM1290020
Links:
External link:
Runs: 1 run, 24.2M spots, 2.4G bases, 1.1Gb
Run# of Spots# of BasesSizePublished
SRR105076324,166,3242.4G1.1Gb2016-03-01

ID:
563543

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