GSM3028061: 241: 4X choline male rep 2 (mRNA); Mus musculus; RNA-Seq - SRA

SRX3753519: GSM3028061: 241: 4X choline male rep 2 (mRNA); Mus musculus; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 45.6M spots, 3.7G bases, 557.4Mb downloads

Submitted by: NCBI (GEO)

Study: An exploratory study to examine the effects of maternal choline supplementation during mouse pregnancy on placental epigenetic markers (mRNA-seq data set)

PRJNA436482 • SRP133742 • All experiments • All runs

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This study sought to explore the effects of 4X maternal choline supplementation (MCS) on mRNA and miRNA abundance in mouse placentas collected on gestational day 15.5. These data advance our understanding about the importance of choline in reproductive health and will guide future mechanistic studies designed to elucidate the mechanisms mediating choline''s beneficial effects. Overall design: mRNA sequencing was performed on 6 placental samples (3 males and 3 females) from 1X control choline diet group and 6 placental samples (3 males and 3 females) from 4X choline diet group

Sample: 241: 4X choline male rep 2 (mRNA)

SAMN08627250 • SRS3009517 • All experiments • All runs

Organism: Mus musculus

Library:

Instrument: NextSeq 500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Placenta tissues were removed from the mice after they were sacrificed at gestational day 15.5 and were immediately flash frozen in liquid nitrogen. RNA were extracted from the tissues using Trizol following the manufacturer's protocol. RNA-seq libraries were prepared from 1 ug total RNA using the NEBNext Directional Ultra RNA Library Prep Kit for Illumina (New England Biolabs), with initial polyA+ isolation.

Experiment attributes:

GEO Accession: GSM3028061

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 45.6M spots, 3.7G bases, 557.4Mb

Run	# of Spots	# of Bases	Size	Published
SRR6794501	45,603,648	3.7G	557.4Mb	2018-04-10

ID:: 5176850

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