Instrument: Illumina HiSeq 4000
Strategy: miRNA-Seq
Source: TRANSCRIPTOMIC
Selection: size fractionation
Layout: SINGLE
Construction protocol: Plasma/Serum RNA Purification Kit using Norgen Biotek (Cat#55000) a standard library preparation workflow using Norgen's Small RNA Library Prep Kit for Illumina (Cat# 63600). Six microliter of each RNA sample was used as input. The workflow includes the following steps in sequence - 3' adapter ligation, 5' adapter ligation, reverse transcription/cDNA synthesis and then the indexing PCR. The final library is then subjected to column clean-up before resolving on a 6% TBE PAGE gel for size selection. The target band (~141-144 bp) is then excised from PAGE gel and purified