GSM2989264: Melanocyte2 10J UVB; Homo sapiens; miRNA-Seq - SRA

SRX3671085: GSM2989264: Melanocyte2 10J UVB; Homo sapiens; miRNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 7.8M spots, 792M bases, 245.8Mb downloads

Submitted by: NCBI (GEO)

Study: Next Generation Sequencing Facilitated Differential Transcriptome Study of melanocytes treated with UVB radiation compared to matched fibroblasts and keratinocytes

PRJNA433554 • SRP132514 • All experiments • All runs

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Transcriptome profiling (mRNA-seq) of Fibroblasts, keratinocytes, melanocytes and melanocytes irradiated with UVB Overall design: mRNA profiles are generated for firbroblasts, keratinocytes, melanocytes and melanocytes irradiated with UVB in triplicates using Illumina's HiSeq 2500 platform

Sample: Melanocyte2 10J UVB

SAMN08502480 • SRS2932392 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: Illumina HiSeq 2500

Strategy: miRNA-Seq

Source: TRANSCRIPTOMIC

Selection: size fractionation

Layout: SINGLE

Construction protocol: Cells were harvested in QiaZol Lysis reagent (Qiagen, Redwood City, CA) at 0h and 24h post-irradiation. RNA was isolated using miRNEasy Isolation kit (Qiagen, Cat. No. 217004) for all melanocyte, fibroblast and keratinocyte samples. RNA libraries were prepared for sequencing using standard Illumina protocols

Experiment attributes:

GEO Accession: GSM2989264

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 7.8M spots, 792M bases, 245.8Mb

Run	# of Spots	# of Bases	Size	Published
SRR6696837	7,841,983	792M	245.8Mb	2020-02-20

ID:: 5076045

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