Instrument: Illumina Genome Analyzer II
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: size fractionation
Layout: SINGLE
Construction protocol: Total RNA was isolated using Trizol (Invitrogen). RNA quantities and quality were assessed using a NanoDrop ND-1000 spectrophotometer or an Agilent 2100 BioanalyzerRNA quantities and quality were assessed using a NanoDrop ND-1000 spectrophotometer or an Agilent 2100 Bioanalyzer. Libraries of small RNAs for sequencing were prepared using the DGE-Small RNA Sample Kit, Alternative v1.5 Protocol (Illumina; San Diego, California) according to the protocol supplied with the reagents (Protocol Rev. A, published February 2009) and using 1ug of total RNA. One lane of each library was sequenced on the Genome Analyzer IIx (Illumina) using the 36 Cycle Sequencing Kit v5 and v4 flowcell and cluster reagents (Catalog FC-104-5020 and GD-300-1001)