GSM2912447: RNA_Sch3 rep3; Plasmodium falciparum; RNA-Seq - SRA

SRX3528874: GSM2912447: RNA_Sch3 rep3; Plasmodium falciparum; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 16.1M spots, 3.3G bases, 1.5Gb downloads

Submitted by: NCBI (GEO)

Study: Genome-wide mapping of RNAPII and its phosphoisoforms in Plasmodium falciparum

PRJNA428361 • SRP127979 • All experiments • All runs

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RNAPII ChIP seq was carried out with various phospho antibodies and compared and correlated with the RNA -seq expression data from the same stage sample Overall design: RNAPII ChIP -seq was carried out with anti-CTD, anti-Ser5-P, anti-Ser2/5-P and N20 antibodies at three different stages, ring, trophozoites and schizonts across the inter-erythrocytic developmental cycle (IDC) of Plasmodium falciparum. RNAPII binding was correlated with mRNA expression levels generated by RNA Seq. RNA- seq experiment includes three biological replicates.

Sample: RNA_Sch3 rep3

SAMN08288502 • SRS2804783 • All experiments • All runs

Organism: Plasmodium falciparum

Library:

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: RNA from each stage was isolated by Trizol method. Dnase treated RNA was used for preparing library for RNA Seq.Lysates were clarified from sonicated nuclei and RNAPII-DNA complexes were isolated with RNAPII antibodies. Library preparation for RNA Seq was performed according to Illumina¹s Stranded mRNA sample preparation protocol, following the manufacturer¹s recommendations. Libraries for ChIP-Seq were prepared using NEBNext Ultra DNA library prep kit from Illumina

Experiment attributes:

GEO Accession: GSM2912447

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 16.1M spots, 3.3G bases, 1.5Gb

Run	# of Spots	# of Bases	Size	Published
SRR6437406	16,149,399	3.3G	1.5Gb	2020-12-30

ID:: 4903801

SRA

Sequence Read Archive

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