Instrument: Illumina HiSeq 2000
Strategy: OTHER
Source: TRANSCRIPTOMIC
Selection: other
Layout: SINGLE
Construction protocol: Cell pellets were thawed and lysed with bead beating. Total RNA was extracted with miRNeasy RNA Extraction Kit (Qiagen). rRNA was subtracted with the Ribozero Gram Negative Kit (Illumina). RNA was then fragmented with heat and alkalinity and size selected between 20 and 40 bases with denaturing PAGE. RNA samples were ligated to a 3' adaptor and reverse transcribed. The cDNA was circularized to add a 5' adaptor then PCR amplified to add sample specific barcodes.