Instrument: Illumina HiSeq 2000
Strategy: OTHER
Source: TRANSCRIPTOMIC
Selection: other
Layout: SINGLE
Construction protocol: RNA was extracted using Trizol (Invitrogen) and Direct-zol RNA MiniPrep kit (Zymo). See publication for complete protocol. Briefly, 5'-P terminated RNA was degraded with terminator exonuclease (Epicentre) and then 5'-OH terminated RNA was phosphorylated with optikinase (USB). An RNA linker containing an amplification region was then ligated onto phosphorylated RNA. Reverse transcription was conducted with a primer with a randomized region as well as an amplification region. Finally, libraries were amplified and sequenced.