GSM2864797: Hep G2 wt cells untreated, biological rep3; Homo sapiens;... - SRA

SRX3421798: GSM2864797: Hep G2 wt cells untreated, biological rep3; Homo sapiens; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 6.1M spots, 449.3M bases, 192.5Mb downloads

Submitted by: NCBI (GEO)

Study: Transcriptome profile of ATP7B KO cells and WT cells treated with Copper (Cu) to study Autophagy as a pro-survival mechanism in Wilson disease

PRJNA419762 • SRP125604 • All experiments • All runs

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To understand whether hepatic cells use autophagy to defend against Cu toxicity in WD, we employed ATP7B knockout (ATP7B-KO) HepG2 cell line, by Quant-seq experiments. Transcriptome analysis of the of ATP7B KO cells and WT cells treated with Copper (Cu) compared with untreated cells. Overall design: total RNA was extracted from ATP7B KO cells and WT cells treated with Copper (Cu); RNA extracted from untreated cells was used as control.

Sample: Hep G2 wt cells untreated, biological rep3

SAMN08097619 • SRS2713561 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: NextSeq 500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Extraction of total RNA was performed according to the manufacturer's instructions (Qiagen). Total RNA (100 ng) from each sample was prepared using QuantSeq 3'mRNA-Seq Library prep kit (Lexogen, Vienna, Austria) according to manufacturer's instructions The amplified fragmented cDNA of 300 bp in size were sequenced in single-end mode using the NextSeq500 (Illumina) with a read length of 75 bp

Experiment attributes:

GEO Accession: GSM2864797

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 6.1M spots, 449.3M bases, 192.5Mb

Run	# of Spots	# of Bases	Size	Published
SRR6321975	6,055,450	449.3M	192.5Mb	2019-02-26

ID:: 4765881

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