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SRX3410335: GSM2862144: 293T EV; Homo sapiens; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 26.7M spots, 8G bases, 3.1Gb downloads

Submitted by: NCBI (GEO)
Study: Whole genome RNA-seq analysis of Wild type or Elf4-/- peritoneal macrophages and whole genome RNA-seq analysis of 293T cells transfected with miR-221 or empty vector.
show Abstracthide Abstract
Wild type or Elf4-/- peritoneal macrophages were infected with VSV, followed by whole genome RNA-seq analysis. 293T cells were transfected with miR-221 or empty vector, followed by whole genome RNA-seq analysis. Conclusions:Overexpression of miR-221 in cells facilitated viral infection and inhibited the production of IFNß. Overall design: Wild type or Elf4-/- peritoneal macrophages were infected with VSV for 4 hours. Cells were harvested followed by whole genome RNA-seq analysis. 293T cells were transfected with miR-221 or empty vector. 6 hours later the cells were were harvested followed by whole genome RNA-seq analysis.
Sample: 293T EV
SAMN08045520 • SRS2703416 • All experiments • All runs
Organism: Homo sapiens
Library:
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Peritoneal macrophages and 293T cells were prepared, and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols
Experiment attributes:
GEO Accession: GSM2862144
Links:
Runs: 1 run, 26.7M spots, 8G bases, 3.1Gb
Run# of Spots# of BasesSizePublished
SRR630995226,677,8788G3.1Gb2017-11-22

ID:
4751341

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