GSM2837647: 09020: NE-Infused 4; Ovis aries; RNA-Seq - SRA

SRX3353299: GSM2837647: 09020: NE-Infused 4; Ovis aries; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 33.4M spots, 6.7G bases, 3.7Gb downloads

Submitted by: NCBI (GEO)

Study: RNAseq of Islets Isolated from Near Term Fetal Sheep after 7-day Infusion of Norepinephrine or Vehicle Control

PRJNA416873 • SRP123451 • All experiments • All runs

show Abstracthide Abstract

The study objective was to determine differentially expressed mRNA transcripts in pancreatic islets from fetal sheep exposed to persistent elevations in stress hormone norepinephrine to isolate effects of chronic adrenergic signaling on fetal islets. Ultimately, these data can be compared to islets from fetuses with placental insufficiency-induced intrauterine growth restriction, where there is known increases in norepinephrine. Overall design: Islets were isolated for high-throughput sequencing from near term sheep fetuses from a group receiving 7-day norepinephrine infusion and a group receiving saline-vehicle control.

Sample: 09020: NE-Infused 4

SAMN07967133 • SRS2652502 • All experiments • All runs

Organism: Ovis aries

Library:

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Islets were isolated and handpicked then frozen at -80C for RNA extraction with Qiagen Microprep RNAeasy mRNA was selected and double-stranded cDNA libraries with ligated sequencing adapters was constructed with the Illumina TruSeq RNA Sample Prep Kit. Cluster generation was conducted with Illumina TruSeq 100bp PE (paired end) cluster kit

Experiment attributes:

GEO Accession: GSM2837647

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 33.4M spots, 6.7G bases, 3.7Gb

Run	# of Spots	# of Bases	Size	Published
SRR6246125	33,418,762	6.7G	3.7Gb	2018-09-06

ID:: 4686237

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