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SRX3333998: GSM2832729: human_ESC_w3_fj105; Homo sapiens; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 48.9M spots, 9.8G bases, 6.5Gb downloads

Submitted by: NCBI (GEO)
Study: Human embryonic stem cell, chimpanzee induced pluripotent stem cell, orangutan induced pluripotent stem cell, rhesus embryonic stem cell, and their derived cortical organoid RNA-seq
PRJNA415990 • SRP121791 • All experiments • All runs
show Abstracthide Abstract
We compared a 5 week time course of cortical organoid differentiation across human, chimpanzee, orangutan, and rhesus using bulk RNAseq. In addition, single cell RNAseq was performed on a subset of time points from human cells in weeks 0, 1, 2, and 5. Overall design: Bulk RNAseq of hESCs, ch-iPSCs, or-iPSCs, rhESCs, and each of 6 time points corresponding to weeks 0, 1, 2, 3, 4, and 5 during cortical organoid differentiation. Single cell RNAseq of human ESCs, week 1, week 2, and week 5 organoids.
Sample: human_ESC_w3_fj105
SAMN07839661 • SRS2636418 • All experiments • All runs
Organism: Homo sapiens
Library:
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Trizol + RNeasy or Direct-zol cleanup, followed by ribozero rRNA depletion. Strand-specific dUTP followed by TruSeq DNA library prep kit.
Experiment attributes:
GEO Accession: GSM2832729
Links:
Runs: 1 run, 48.9M spots, 9.8G bases, 6.5Gb
Run# of Spots# of BasesSizePublished
SRR622539048,924,5879.8G6.5Gb2018-05-31

ID:
4663127

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