GSM2829447: H3.1; Arabidopsis thaliana; ChIP-Seq - SRA

SRX3316636: GSM2829447: H3.1; Arabidopsis thaliana; ChIP-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 30.8M spots, 1.6G bases, 498.9Mb downloads

Submitted by: NCBI (GEO)

Study: Plant-specific histone residue F41 restricts H3.1 distribution in heterochromatin

PRJNA360334 • SRP096152 • All experiments • All runs

show Abstracthide Abstract

The plant specific H3.1F41 plays crucial role in H3.1 genome-wide deposition pattern. Overall design: We made single and double mutant transgenic lines between histone 3 variants H3.3 and canonical H3.1 in Arabidopsis. The mutated constracts were fused with FLAG tag and transformed into Arabidopsis driven by their endougenous promoter. ChIP seq experiments were did by anti FLAG tag (Sigma (M8823)).

Sample: H3.1

SAMN07829634 • SRS2622208 • All experiments • All runs

Organism: Arabidopsis thaliana

Library:

Instrument: Illumina HiSeq 2000

Strategy: ChIP-Seq

Source: GENOMIC

Selection: ChIP

Layout: SINGLE

Construction protocol: ChIP genomic DNA protocols are followed by Zhong et al., Nat Struct Mol Biol, 2012, 19:870-850. ChIP library was construbuted by using the Ovation Ultralow Library Kit (NuGen, Part No. 0330).

Experiment attributes:

GEO Accession: GSM2829447

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 30.8M spots, 1.6G bases, 498.9Mb

Run	# of Spots	# of Bases	Size	Published
SRR6207345	30,772,123	1.6G	498.9Mb	2018-01-02

ID:: 4645373

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