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SRX3268646: GSM2808010: transported_miRNA seq; Anas platyrhynchos; miRNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 9.5M spots, 473.4M bases, 225.1Mb downloads

Submitted by: NCBI (GEO)
Study: miRNA profile of duck duodenum mucosa damage caused by transportation stress
show Abstracthide Abstract
We deep sequenced and analyzed miRNAs using deep RNA sequencing (RNA-seq) in transported and control duck's duodenum sample of Jingjiang duck. We analyzed the miRNA data with 9467248 reads and 9808143 million reads, obtained 9338224 and 9677777 clean reads in transported and control duck's by high-throughput sequencing, respectively. we respectively gained 4636135 and 4759049 miRNAs sequences in two groups by filtering the known non-miRNA reads, such as rRNA, tRNA, snRNA, and snoRNA by screening against ncRNA deposited in the GenBank and Rfam databases. These findings provided insights into the expression profiles of miRNAs in duck duodenum, and deepened our understanding of miRNAs in transportation injury of duck. Overall design: Examination of miRNA expression profile in transported duck's duodenum and control duck's duodenum samples
Sample: transported_miRNA seq
SAMN07768662 • SRS2580415 • All experiments • All runs
Library:
Instrument: Illumina HiSeq 2000
Strategy: miRNA-Seq
Source: TRANSCRIPTOMIC
Selection: size fractionation
Layout: SINGLE
Construction protocol: Total RNA of each sample was extracted with Trizol (Invitrogen, USA). Using a Novex 15% TBE-Urea gel, 20–30-nt long RNA fragments were isolated and purified from 10 μg of total RNA RNA libraries were prepared for sequencing using standard Illumina protocols
Experiment attributes:
GEO Accession: GSM2808010
Links:
Runs: 1 run, 9.5M spots, 473.4M bases, 225.1Mb
Run# of Spots# of BasesSizePublished
SRR61568159,467,248473.4M225.1Mb2019-01-01

ID:
4585964

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