GSM2793714: input control Pol2 rep1; Homo sapiens; Bisulfite-Seq - SRA

SRX3216476: GSM2793714: input control Pol2 rep1; Homo sapiens; Bisulfite-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 50.9M spots, 2.5G bases, 1Gb downloads

Submitted by: NCBI (GEO)

Study: DNA methylation profiling of TF bound sites using ChIPBS (ChIP Bisulfite Sequencing)

PRJNA412696 • SRP119183 • All experiments • All runs

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ChIPBS analysis of HepG2 cells was performed using more direct assay approach, to study the methylation status of transcription factor bound sites and elucidate our understanding on TF-DNA methylation crosstalk Overall design: ChIP followed by Bisulfite sequencing were performed in HepG2 cell lines. Methylation profile was measured for JUND, YY1, ZBTB33, POL2R and TAF1 and compared to the wgbs methylation profile for the same TF bound sites.

Sample: input control Pol2 rep1

SAMN07702243 • SRS2541551 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: Illumina HiSeq 2500

Strategy: Bisulfite-Seq

Source: GENOMIC

Selection: RANDOM

Layout: SINGLE

Construction protocol: Cross-linked chromatin was immunoprecipitated with an antibody. The Protein:DNA crosslinks were then reversed and the DNA fragments were recovered and sequenced. Please see protocol notes below and go to http://hudsonalpha.org/myers-lab/protocols for the most current version of the protocol. The eluted DNA was followed by bisulfite conversion using the EZ DNA Methylation-Gold Kit from Zymo according to manufacturer’s instructions. Illumina Single End for ChIPBS

Experiment attributes:

GEO Accession: GSM2793714

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 50.9M spots, 2.5G bases, 1Gb

Run	# of Spots	# of Bases	Size	Published
SRR6076559	50,870,376	2.5G	1Gb	2021-12-31

ID:: 4525470

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