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SRX3213622: GSM2792515: 489_039; Bos taurus; RNA-Seq
1 ILLUMINA (Illumina Genome Analyzer II) run: 6.7M spots, 719.6M bases, 382Mb downloads

Submitted by: NCBI (GEO)
Study: Transcriptional response of bovine moncocyte-derived macrophages to infection with strains of Mycobacterium bovis and Mycobacterium avium subspecies paratuberculosis
show Abstracthide Abstract
Mycobacterium bovis (M. bovis) and Mycobacterium avium subspecies paratuberculosis (MAP) are important pathogens of cattle, causing bovine tuberculosis and Johne's disease respectively. M. bovis and MAP infect residential macrophages in the lung and intestines respectively and subvert the macrophage biology to create a survival niche. To investigate this interaction we simultaneously studied the transcriptional response of bovine monocyte-derived macrophages to infection with two strains of M. bovis (AF2122/97 and G18) and two strains of MAP (C & L1). Overall design: 120 samples were analysed in total; derived from six biological replicates (i.e. cells isolated from six cattle). Cells were left uninfected (medium only controls) or infected with either a M. bovis strain (AF2122/97 or G18) or a MAP strain (C or L1). Cells were harvested at 2, 6, 24 and 48h post infection.
Sample: 489_039
SAMN07693443 • SRS2538378 • All experiments • All runs
Organism: Bos taurus
Library:
Instrument: Illumina Genome Analyzer II
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: At the desired time point the cells were lysed with 2.5 ml Trizol reagent (Invitrogen) for 20 minutes, to ensure mycobacterial inactivation. RNA was extracted from the samples following the manufacturer's instructions. mRNA was purified from the total RNA and fractionated to generate fragments with a medium size of 180-210 nucleotides. These fragments were reverse transcribed using random primers to generate cDNA libraries.
Experiment attributes:
GEO Accession: GSM2792515
Links:
Runs: 1 run, 6.7M spots, 719.6M bases, 382Mb
Run# of Spots# of BasesSizePublished
SRR60720856,663,290719.6M382Mb2017-10-11

ID:
4522441

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