Instrument: Illumina HiSeq 2000
Strategy: ChIP-Seq
Source: GENOMIC
Selection: ChIP
Layout: SINGLE
Construction protocol: Input and ChIP DNA were sent for sequencing. All samples were prepared using a liquid handling robot (Hamilton) by Bianka Baying (EMBL, Heidelberg, Germany). 10ng of each sample mixed with library preparation were assessed according to manufacturer's recommendation (NEBNext Ultra™ DNA Library Prep Kit for Illumina, (#E7370S/L)). Library preparation was done according to manufacturer. Briefly, the Adapter in the Adapter Ligation step was diluted 1:30 in water. The size selection step was performed with Ampure XP Beads from Beckman Coulter (insert size 200-250bp). The PCR amplification was performed in 15 cycles. Finally NEBNext Multiplex Oligos for Illumina (Index Primer Set 1) (#E7335S/L) were used for the Indexing. The quality and quantity of the libraries was checked using the Agilent BioAnalyzer and the Qubit dsDNA HS Assay kit (Invitrogen). ChIP-Seq was performed using Illumina HiSeq 2000 Sequencer with Requested Read Length HiSeq50 SE.