BISULFITE_CONVERSION_PROTOCOL: 2x5hrs Epitect Kit
DNA_PREPARATION_INITIAL_DNA_QNTY: 5 ug
EXTRACTION_PROTOCOL_SONICATION_CYCLES:
Covaris shearing, duty cycle 5%, intensity 5, cycl (show full text...)(hide...)Covaris shearing, duty cycle 5%, intensity 5, cycles / burst 200, duration 9 minutes
DNA_PREPARATION_ADAPTOR_LIGATION_PROTOCOL: T4 Ligase (2,000U/ul) 16C o/n
DNA_PREPARATION_ADAPTOR_SEQUENCE: Illumina paired end adapter
LIBRARY_GENERATION_PCR_R_PRIMER_SEQUENCE: PE1.0
DNA_PREPARATION_POST-LIGATION_FRAGMENT_SIZE_SELECTION: 250-400
BISULFITE_CONVERSION_PERCENT: 99.5%
EXTRACTION_PROTOCOL:
Standard Protocol (Smith et al., Methods 48, 226-2 (show full text...)(hide...)Standard Protocol (Smith et al., Methods 48, 226-232)
LIBRARY_GENERATION_PCR_PRIMER_CONC: 25uM
DNA_PREPARATION_FRAGMENT_SIZE_RANGE: 130-280
EXPERIMENT_TYPE: DNA Methylation
LIBRARY_GENERATION_PCR_THERMOCYCLING_PROGRAM: Smith et al., Methods 48, 226-232
LIBRARY_GENERATION_PCR_PRODUCT_ISOLATION_PROTOCOL: SPRI Beads
LIBRARY_GENERATION_PCR_F_PRIMER_SEQUENCE: PE2.0
LIBRARY_GENERATION_PCR_TEMPLATE_CONC: NA
LIBRARY_GENERATION_PCR_POLYMERASE_TYPE: Pfu Turbo Cx hot start
LIBRARY_GENERATION_PCR_NUMBER_CYCLES: 5-8
EXTRACTION_PROTOCOL_TYPE_OF_SONICATOR: Covaris S2 (TM)
GEO Accession: GSM1172596