GSM2740541: 2142_MWF; Rattus norvegicus; RNA-Seq - SRA

SRX3088607: GSM2740541: 2142_MWF; Rattus norvegicus; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 57.9M spots, 9.2G bases, 3.1Gb downloads

Submitted by: NCBI (GEO)

Study: Integrative analysis of the genomic architecture of a complex kidney damage QTL in inbred hypertensive rats implies Tmem63c for translational research

PRJNA398012 • SRP115283 • All experiments • All runs

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Despite recent advances in genomics, the identification of genes underlying complex diseases remains challenging. Inbred rat strains offer well-established experimental models to support the identification of susceptibility genes for diseases such as kidney damage associated with elevated blood pressure or hypertension. Here, we combined quantitative trait locus (QTL) mapping in two contrasting inbred rat strains with targeted next generation sequencing (NGS), compartment-specific transcriptome sequencing (RNA-Seq), and phenotype directed follow-up translational analysis. Overall design: mRNA profiles of spontaneously hypertensive rats and Munich Wistar Frömter rats were generated by deep sequencing, in triplicate, using NextSeq 500

Sample: 2142_MWF

SAMN07499369 • SRS2426337 • All experiments • All runs

Organism: Rattus norvegicus

Library:

Instrument: NextSeq 500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Total RNA of glomeruli preparations was isolated and DNase-treated by RNeasy Purification Kits (Qiagen) The NEBNext Poly(A) mRNA magnetic isolation module followed by library preparation using NEBNext Ultra RNA Library Prep Kit for Illumina (New England BioLabs) was applied on 1 µg total RNA to generate a cDNA library

Experiment attributes:

GEO Accession: GSM2740541

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 57.9M spots, 9.2G bases, 3.1Gb

Run	# of Spots	# of Bases	Size	Published
SRR5928288	57,893,200	9.2G	3.1Gb	2018-08-12

ID:: 4378312

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