U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

SRX3082737: GSM2738066: Input DNA in lytic cells; Homo sapiens; Human gammaherpesvirus 8; ChIP-Seq
1 ILLUMINA (Illumina HiSeq 3000) run: 8.7M spots, 436.2M bases, 198Mb downloads

Submitted by: NCBI (GEO)
Study: ZIC2 ChIP-Seq in KSHV latent and lytic reactivated BCBL-1 cells
PRJNA397777 • SRP115146 • All experiments • All runs
show Abstracthide Abstract
ZIC2 is required for the maintenance of KSHV latency in human host cells. To understand the molecular action of ZIC2, we map ZIC2 binding sites across the KSHV genome in BCBL-1 cells using ChIP-Seq analysis. Overall design: Examination of ZIC2 binding in KSHV latent and lytic reactivated cells
Sample: Input DNA in lytic cells
SAMN07487361 • SRS2421375 • All experiments • All runs
Organism: Homo sapiens
Library:
Instrument: Illumina HiSeq 3000
Strategy: ChIP-Seq
Source: GENOMIC
Selection: ChIP
Layout: SINGLE
Construction protocol: DNA was sonicated to an average size of ~300 bp using a Diagenode Bioruptor. Chromatin DNA from 1 X 108 cells was used per immunoprecipitation assay with 10 µg of ZIC2 antibody ChIP-enriched and input DNA samples were used to generate Illumina-compatible libraries with the KAPA LTP Library Preparation Kit (Kapa Biosystems, KR0453) according to manufacturer’s recommendations.
Experiment attributes:
GEO Accession: GSM2738066
Links:
Runs: 1 run, 8.7M spots, 436.2M bases, 198Mb
Run# of Spots# of BasesSizePublished
SRR59220838,724,714436.2M198Mb2017-08-25

ID:
4367012

Supplemental Content

Related information

Search details

See more...

Recent activity

Clear Turn Off Turn On

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...