GSM2650868: CLL-01 COCULTURE; Homo sapiens; RNA-Seq - SRA

SRX2885658: GSM2650868: CLL-01 COCULTURE; Homo sapiens; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 66.1M spots, 13G bases, 8.3Gb downloads

Submitted by: NCBI (GEO)

Study: Gene expression activation in CLL mediated by MSCs contact

PRJNA389433 • SRP108685 • All experiments • All runs

show Abstracthide Abstract

Survival of primary CLL cells is mediated by mesenchimal stromal cells in vitro. The goal of this study is to compare the gene expression profile of primary CLL cells in monoculture to that one following cell-cell contact with MSCs Overall design: Primary CLL cells isolated from 6 different patients were cultured for 4h in normal growing medium or for 48h in coculture with EL08-1d1 cells

Sample: CLL-01 COCULTURE

SAMN07198476 • SRS2253709 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Total RNA was extracted by using the RNeasy Mini Kit (Qiagen). Total-RNA concentration and purity of total-RNA samples was measured with the NanoDrop 1000 spectrophotometer (NanoDrop Technologies/Thermo Scientific). Libraries were prepared for sequencing using standard illumina protocols

Experiment attributes:

GEO Accession: GSM2650868

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 66.1M spots, 13G bases, 8.3Gb

Run	# of Spots	# of Bases	Size	Published
SRR5648292	66,109,196	13G	8.3Gb	2019-05-20

ID:: 4133456

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