GSM2648656: ctrl-ASO-dose2-rep2; Mus musculus; RNA-Seq - SRA

SRX2880572: GSM2648656: ctrl-ASO-dose2-rep2; Mus musculus; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 10.3M spots, 749.9M bases, 350.3Mb downloads

Submitted by: NCBI (GEO)

Study: Liver DGE analysis of hemophilia mice (hFIX9-R29X) treated with ASOs inhibiting NMD

PRJNA388971 • SRP108495 • All experiments • All runs

show Abstracthide Abstract

To evaluate the impact of ASO-mediated depletion of NMD factor Upf1 and Upf3b on the normal transcriptome, we performed digital gene expression analysison hFIX-R29X mouse liver treated with Upf3b-GalNAc-ASO. Overall design: hFIX-R29X mice 8-14 weeks of age (n=5-6, 2-3 female and 2-3 male mice per group) were treated every 5 days with 6 doses of DPBS, Control-GalNAc-ASO (15 or 20mg/kg), Upf1-GalNAc-ASO (20 mg/kg) and Upf3b-GalNAc-ASO (10 or 20 mg/kg). Animals were sacrificed 48 h after the last dose.

Sample: ctrl-ASO-dose2-rep2

SAMN07189767 • SRS2248438 • All experiments • All runs

Organism: Mus musculus

Library:

Instrument: NextSeq 500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Animal tissues were homogenized in guanidine isothiocyanate solution (Invitrogen) supplemented with 8% 2-mercaptoethanol (Sigma-Aldrich). Total RNA was prepared using the RNeasy mini Kit (Qiagen). Digital gene expression (DGE) was performed by sequencing fragment libraries from purified total RNA as described previously (Patro et al., Nat Methods, 2017) using the QuantSeq 3' mRNA-Seq Library Prep Kit from Lexogen

Experiment attributes:

GEO Accession: GSM2648656

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 10.3M spots, 749.9M bases, 350.3Mb

Run	# of Spots	# of Bases	Size	Published
SRR5642386	10,255,125	749.9M	350.3Mb	2017-12-06

ID:: 4123595

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