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SRX2880571: GSM2648655: ctrl-ASO-dose2-rep1; Mus musculus; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 1.7M spots, 123.1M bases, 54.8Mb downloads

Submitted by: NCBI (GEO)
Study: Liver DGE analysis of hemophilia mice (hFIX9-R29X) treated with ASOs inhibiting NMD
PRJNA388971 • SRP108495 • All experiments • All runs
show Abstracthide Abstract
To evaluate the impact of ASO-mediated depletion of NMD factor Upf1 and Upf3b on the normal transcriptome, we performed digital gene expression analysison hFIX-R29X mouse liver treated with Upf3b-GalNAc-ASO. Overall design: hFIX-R29X mice 8-14 weeks of age (n=5-6, 2-3 female and 2-3 male mice per group) were treated every 5 days with 6 doses of DPBS, Control-GalNAc-ASO (15 or 20mg/kg), Upf1-GalNAc-ASO (20 mg/kg) and Upf3b-GalNAc-ASO (10 or 20 mg/kg). Animals were sacrificed 48 h after the last dose.
Sample: ctrl-ASO-dose2-rep1
SAMN07189768 • SRS2248437 • All experiments • All runs
Organism: Mus musculus
Library:
Instrument: NextSeq 500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Animal tissues were homogenized in guanidine isothiocyanate solution (Invitrogen) supplemented with 8% 2-mercaptoethanol (Sigma-Aldrich). Total RNA was prepared using the RNeasy mini Kit (Qiagen). Digital gene expression (DGE) was performed by sequencing fragment libraries from purified total RNA as described previously (Patro et al., Nat Methods, 2017) using the QuantSeq 3' mRNA-Seq Library Prep Kit from Lexogen
Experiment attributes:
GEO Accession: GSM2648655
Links:
Runs: 1 run, 1.7M spots, 123.1M bases, 54.8Mb
Run# of Spots# of BasesSizePublished
SRR56423851,679,644123.1M54.8Mb2017-12-06

ID:
4123594

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