GSM2643986: WT-2 Adenoma; Mus musculus; RNA-Seq - SRA

SRX2869206: GSM2643986: WT-2 Adenoma; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 69.2M spots, 3.5G bases, 1.2Gb downloads

Submitted by: NCBI (GEO)

Study: AP4 regulates stem and Paneth cell homeostasis and promotes adenoma initiation in the intestine (adenoma)

PRJNA388457 • SRP108280 • All experiments • All runs

show Abstracthide Abstract

AP4 is frequently expressed in primary CRCs. However, the in vivo relevance of AP4 for development of intestinal tumor formation has not been analyzed by genetic approaches. ApcMin/+ mice with deletion of AP4 were generated and analyzed. The mRNA expression profiles of intestinal adenomas with and without functional AP4 were compared. Overall design: AP4 deficient ApcMin/+ mice and wild-type ApcMin/+ mice were sacrificed at 16 weeks of age. 3 tumor RNA samples were obtained for each genotype; each tumor RNA sample represented a pool of 5 tumors isolated from the same mouse.

Sample: WT-2 Adenoma

SAMN07177416 • SRS2239580 • All experiments • All runs

Organism: Mus musculus

Library:

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Adenomas were isolated from small intestine, flash frozen into liquid nitrogen, and RNA was harvested using RNeasy Mini Kit (Qiagen). 1 ug of total RNA was used for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols

Experiment attributes:

GEO Accession: GSM2643986

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 69.2M spots, 3.5G bases, 1.2Gb

Run	# of Spots	# of Bases	Size	Published
SRR5628709	69,162,898	3.5G	1.2Gb	2018-12-27

ID:: 4109487

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