show Abstracthide AbstractTo elucidate the molecular bases for the infertile phenotype of Zmym3 KO mice at the mRNA expression level, we carried out RNA sequencing (RNA-seq) analysis on cultured spermatogonial stem cells (SSCs), c-KIT+ pre-meiotic cells induced from SSCs and spermatocytes directly isolated from adult mouse testes. Overall design: The cells used for RNA-Seq analysis including SSCs from Zmym3 WT and KO mice (SSC:WT and SSC:KO), c-KIT+ pre-meiotic cells induced from SSCs (c-KIT+ WT and c-KIT+:KO) and spermatocytes from 5- and 7-month mice (5m-SC:WT, 5m-SC:KO, 7m-SC:WT and 7m-SC:KO).