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SRX2834257: GSM2631948: Zmym3 KO SSC replicate 1; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 4000) run: 28.1M spots, 8.4G bases, 3.2Gb downloads

Submitted by: NCBI (GEO)
Study: Gene knockout of Zmym3 in mice arrests spermatogenesis at meiotic metaphase with defects in spindle assembly checkpoint
show Abstracthide Abstract
To elucidate the molecular bases for the infertile phenotype of Zmym3 KO mice at the mRNA expression level, we carried out RNA sequencing (RNA-seq) analysis on cultured spermatogonial stem cells (SSCs), c-KIT+ pre-meiotic cells induced from SSCs and spermatocytes directly isolated from adult mouse testes. Overall design: The cells used for RNA-Seq analysis including SSCs from Zmym3 WT and KO mice (SSC:WT and SSC:KO), c-KIT+ pre-meiotic cells induced from SSCs (c-KIT+ WT and c-KIT+:KO) and spermatocytes from 5- and 7-month mice (5m-SC:WT, 5m-SC:KO, 7m-SC:WT and 7m-SC:KO).
Sample: Zmym3 KO SSC replicate 1
SAMN07141716 • SRS2208642 • All experiments • All runs
Organism: Mus musculus
Library:
Instrument: Illumina HiSeq 4000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Total RNA from mouse testis cells and mSSCs was extracted using Trizol. The RNA libraries were constructed by Illumina library preparation protocols. High-throughput sequencing was performed on HiSeq4000/HiSeq X 10. RNA libraries were prepared for sequencing using standard Illumina protocols.
Experiment attributes:
GEO Accession: GSM2631948
Links:
Runs: 1 run, 28.1M spots, 8.4G bases, 3.2Gb
Run# of Spots# of BasesSizePublished
SRR557601128,077,2558.4G3.2Gb2017-05-22

ID:
4070535

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