GSM2596615: 335 salt-stress rep1; Oryza sativa; RNA-Seq - SRA

SRX2776308: GSM2596615: 335 salt-stress rep1; Oryza sativa; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 17.3M spots, 1.7G bases, 611.4Mb downloads

Submitted by: NCBI (GEO)

Study: Variation in transcriptional responses to salt stress in rice

PRJNA385135 • SRP106054 • All experiments • All runs

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The aim of this study is to assess natural variation in transcriptional responses to salt stress in rice. We utilized a diversity panel (RDP1) described in Zhao et al 2011. Eight day old rice seedlings were subjected to a gradual 6 dS·m-1 salt stress for a period of 24h. RNA seqeuncing was performed on shoot tissue using Illumina HiSeq 2500. Overall design: RNA deep sequencing

Sample: 335 salt-stress rep1

SAMN06860970 • SRS2159566 • All experiments • All runs

Organism: Oryza sativa

Library:

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: The samples were ground with Tissuelyser II (Invitrogen) and total RNA was isolated with RNAeasy isolation kit (Qiagen) according to manufacturer’s instructions. On-column DNase treatment was performed to remove genomic DNA contamination (Qiagen). 96 libraries were prepared using TruSeq RNA v2 library preparation reagents. Eighteen samples were sequenced per lane.

Experiment attributes:

GEO Accession: GSM2596615

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 17.3M spots, 1.7G bases, 611.4Mb

Run	# of Spots	# of Bases	Size	Published
SRR5495351	17,280,118	1.7G	611.4Mb	2018-01-02

ID:: 4004281

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