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SRX2754560: GSM2586926: 24hInd + 0.5mM PFA ORF59 ChIP (iSLK-Bac16WT cells); Human gammaherpesvirus 8; ChIP-Seq
1 ILLUMINA (NextSeq 500) run: 113,625 spots, 8.6M bases, 2.7Mb downloads

Submitted by: NCBI (GEO)
Study: Identification of symmetric methylation of histone H4 arginine 3 on KSHV genome (iSLKBac16WT ChIP-Seq)
PRJNA383982 • SRP105042 • All experiments • All runs
show Abstracthide Abstract
The objective of this study was to identify the symmetric methylation sites on KSHV genome during latency and lytic reactivation Overall design: KSHV infected iSLKBac16WT cells were induced with doxycycline for 24h to trigger the lytic reactivation, cells were crosslinked and subjected for chromatin isolation and sonication. ChIP assay was performed using specific antibodies.
Sample: 24hInd + 0.5mM PFA ORF59 ChIP (iSLK-Bac16WT cells)
SAMN06820781 • SRS2139813 • All experiments • All runs
Organism: Human gammaherpesvirus 8
Library:
Instrument: NextSeq 500
Strategy: ChIP-Seq
Source: GENOMIC
Selection: ChIP
Layout: SINGLE
Construction protocol: Latent and lytic cells were cross-linked using formaldehyde to fix the protein-DNA for ChIP assay Chromatins were immunoprecipitated using specific listed antibody by following standard ChIP protocol ChIP DNA was used for library prepartation for ChIP-seq analysis
Links:
Runs: 1 run, 113,625 spots, 8.6M bases, 2.7Mb
Run# of Spots# of BasesSizePublished
SRR5469235113,6258.6M2.7Mb2017-04-27

ID:
3973645

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