GSM2501675: WT flies grown in 18C replica 3; Drosophila melanogaster... - SRA

SRX2585872: GSM2501675: WT flies grown in 18C replica 3; Drosophila melanogaster; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 10.4M spots, 781.2M bases, 342.2Mb downloads

Submitted by: NCBI (GEO)

Study: 3'' RNA-seq of ADAR hypomorph flies grown in different temperatures

PRJNA376607 • SRP100647 • All experiments • All runs

show Abstracthide Abstract

In order to test whether ADAR is responsible for mediating part of the transcriptional response to temperature changes in fly we determined the gene expression profile of fly heads from control and ADAR Hypomorph flies at 18 and 29°C by 3' RNA-seq. Overall design: ADAR hypomorph flies and the control flies were grown in 18C (3 replicas each) or in 29C (3 replicas each) and harvested 3-6 days after eclosion .

Sample: WT flies grown in 18C replica 3

SAMN06439454 • SRS1999536 • All experiments • All runs

Organism: Drosophila melanogaster

Library:

Instrument: NextSeq 500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Total RNA was extracted using Trizol reagent (Sigma) and treated with DNase I (NEB) following the manufacturer's protocol. Libraries preparation was based on a method previously described (Engreitz et al., 2013). The protocol was modified to get only 3' ends of the genes by applying polyA selection step after fragmentation and first linker ligation.

Experiment attributes:

GEO Accession: GSM2501675

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 10.4M spots, 781.2M bases, 342.2Mb

Run	# of Spots	# of Bases	Size	Published
SRR5282226	10,415,417	781.2M	342.2Mb	2017-07-20

ID:: 3747411

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