Instrument: NextSeq 500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Total RNA was extracted using Trizol reagent (Sigma) and treated with DNase I (NEB) following the manufacturer's protocol. Libraries preparation was based on a method previously described (Engreitz et al., 2013). The protocol was modified to get only 3' ends of the genes by applying polyA selection step after fragmentation and first linker ligation.