GSM2436488: RT4 GSK343 rep 2_7days; Homo sapiens; RNA-Seq - SRA

SRX2439448: GSM2436488: RT4 GSK343 rep 2_7days; Homo sapiens; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 72.7M spots, 11G bases, 6.3Gb downloads

Submitted by: NCBI (GEO)

Study: Transcriptome analysis for KDM6A mutated urothelial bladder carcinoma and EZH2 inhibitor treated KDM6A mutated urothelial bladder carcinoma

PRJNA358425 • SRP095491 • All experiments • All runs

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Purpose: The goals of this study are to compare 1. The transcription profile in KDM6A wildtype and KDM6A mutated urothelial bladder carcinoma. 2. The transcriptional changes in KDM6A mutated urothelial bladder carcinoma upon EZH2 inhibitor treatment. Overall design: Transcriptome profiling of KDM6A wildtype and KDM6A mutated urothelial bladder carcinoma were generated by deep sequencing, 10 cases in each group, using Hiseq2000. Transcriptome profiling of EZH2 inhibitor-treated KDM6A wildtype and KDM6A mutated urothelial bladder carcinoma were generated by deep sequencing, in triplicate, using Hiseq2000.

Sample: RT4 GSK343 rep 2_7days

SAMN06173527 • SRS1874064 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Retinas were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols

Experiment attributes:

GEO Accession: GSM2436488

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 72.7M spots, 11G bases, 6.3Gb

Run	# of Spots	# of Bases	Size	Published
SRR5124463	72,675,610	11G	6.3Gb	2017-03-01

ID:: 3542805

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