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SRX24044159: GSM8164449: WT_MouseNo_2; feces metagenome; OTHER
1 ILLUMINA (Illumina HiSeq 4000) run: 18.8M spots, 5.6G bases, 1.7Gb downloads

External Id: GSM8164449_r1
Submitted by: Fudan University
Study: mWGS analysis of the fecal microbiota of WT and TCRb-/- mice
PRJNA1091644 • SRP497660 • All experiments • All runs
show Abstracthide Abstract
The gut microbiota plays an important role in host health. Microbiota dysbiosis has been implicated in the global epidemic of Metabolic Syndrome (MetS) and could impair host metabolism by noxious metabolites. It has been well established that the gut microbiota is shaped by host immune factors. However, the effect of T cells on the gut microbiota is yet unknown. Here, we performed a metagenomic whole-genome shotgun sequencing (mWGS) study of the microbiota of TCRb-/- mice, which lack alpha/beta T cells. Overall design: Feces were collected from of Tcrb-/- and WT mice. Genomic DNA was extracted and sequenced by a metagenomic whole-genome shotgun sequencing (mWGS).
Sample: WT_MouseNo_2
SAMN40603456 • SRS20836532 • All experiments • All runs
Organism: feces metagenome
Library:
Name: GSM8164449
Instrument: Illumina HiSeq 4000
Strategy: OTHER
Source: GENOMIC
Selection: other
Layout: PAIRED
Construction protocol: Feces were collected and DNA was extracted by QIAGEN Power Fecal Pro DNA Kit following manufacturer's instruction. mWGS DNA library was constructed using TruSeq DNA Library Prep Kit under manufacturer's instruction for metagenomic whole-genome shotgun sequencing (mWGS)
Runs: 1 run, 18.8M spots, 5.6G bases, 1.7Gb
Run# of Spots# of BasesSizePublished
SRR2844033918,798,0995.6G1.7Gb2024-05-06

ID:
32351255

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