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SRX23801373: GSM8121277: ref_a; Aspergillus niger; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 13M spots, 639.2M bases, 229.7Mb downloads

External Id: GSM8121277_r1
Submitted by: westerdijkinstitute
Study: Compare transcriptome profile of reference strain and mutant of HapX during A.niger grown on maltose
show Abstracthide Abstract
Transcriptome analysis was performed to identify the differentially expressed genes between hapX mutant and reference strains during their growth on maltose Overall design: The strains were pre-grown in complete medium containing 2% (w/v) fructose for 16h. The mycelium was harvested and washed with minimal medium (MM) , and then was transferred to 50 ml MM (pH 6) with 25 mM maltose. After 2h of incubation, the mycelium was harvested and frozen in liquid nitrogen. RNA was extracted from grinded mycelia and were single-end sequenced using Illumina HiSeqTM 2000 platform . The reads were mapped to the genome of Aspergillus niger NRRL3 (https://mycocosm.jgi.doe.gov/Aspni_NRRL3_1),
Sample: ref_a
SAMN40208943 • SRS20624363 • All experiments • All runs
Library:
Name: GSM8121277
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: RNA was extracted from grinded mycelia and were single-end sequenced using Illumina HiSeqTM 2000 platform . Purification of mRNA, synthesis of cDNA library and sequencing were conducted at the BGI Tech Solutions Co., Ltd. (Hong Kong, China).
Runs: 1 run, 13M spots, 639.2M bases, 229.7Mb
Run# of Spots# of BasesSizePublished
SRR2817122613,045,782639.2M229.7Mb2024-05-17

ID:
32098817

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