GSM8093357: Liver, Control, rep1; Mus musculus; RNA-Seq - SRA

SRX23689667: GSM8093357: Liver, Control, rep1; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina NovaSeq 6000) run: 20.6M spots, 2.1G bases, 632.8Mb downloads

External Id: GSM8093357_r1

Submitted by: Metabolic Research Laboratories, University of Cambridge

Study: Expression profiling of mouse livers with inducible over-expression of microRNA Mir483

PRJNA1078900 • SRP490879 • All experiments • All runs

show Abstracthide Abstract

In this study we generated mice with inducible over-expression of microRNA Mir483, using a transgene inserted at the Rosa26 locus. Whole transcriptome analysis was performed by RNA-seq in livers collected from 15-week old adult mice (over-expressors and controls). Overall design: We performed RNA-seq analysis in 15-week old mouse livers of adult females with inducible over-expression of microRNA Mir483 (n=6 biological replicates) and littermate controls (n=6 biological replicates)

Sample: Liver, Control, rep1

SAMN40025781 • SRS20520017 • All experiments • All runs

Organism: Mus musculus

Library:

Name: GSM8093357

Instrument: Illumina NovaSeq 6000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Total RNA was extracted using a miRNeasy Mini Kit (Qiagen – 217004), according to manufacturer's protocol. Quantity and quality of RNA were assessed using an Agilent 2100 Bioanalyzer and RNA 6000 Nano Kits (Agilent – 5067-1511) 500 ng total RNA per sample was used to prepare libraries using Illumina's TruSeq RNA Library kit, according to manufacturer's protocol.

Runs: 1 run, 20.6M spots, 2.1G bases, 632.8Mb

Run	# of Spots	# of Bases	Size	Published
SRR28038230	20,620,682	2.1G	632.8Mb	2024-02-26

ID:: 31974051

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