GSM8093337: Liver, wild-type, rep3 (RNA-seq); Mus musculus; RNA-Seq - SRA

SRX23689647: GSM8093337: Liver, wild-type, rep3 (RNA-seq); Mus musculus; RNA-Seq
1 ILLUMINA (Illumina NovaSeq 6000) run: 19.8M spots, 2G bases, 631.8Mb downloads

External Id: GSM8093337_r1

Submitted by: Metabolic Research Laboratories, University of Cambridge

Study: Expression profiling of mouse livers with deletion of paternally-expressed, imprinted Mir483 [RNA-seq]

PRJNA1078902 • SRP490877 • All experiments • All runs

show Abstracthide Abstract

In this study we generated mice with constitutive deletion of microRNA Mir483, which is imprinted and expressed from the paternal allele only. Whole transcriptome analysis was performed by RNA-seq in livers collected from E18.5 fetuses (mutants with deletion of paternal allele - Mir483Pat-KO - and wild-type controls). Overall design: We performed RNA-seq analysis in E18.5 mouse livers of female fetuses with deletion of paternally-expressed microRNA Mir483 (n=5 biological replicates) and wild-type littermate controls (n=5 biological replicates)

Sample: Liver, wild-type, rep3 (RNA-seq)

SAMN40024835 • SRS20519997 • All experiments • All runs

Organism: Mus musculus

Library:

Name: GSM8093337

Instrument: Illumina NovaSeq 6000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Total RNA was extracted using a miRNeasy Mini Kit (Qiagen – 217004), according to manufacturer's protocol. Quantity and quality of RNA were assessed using an Agilent 2100 Bioanalyzer and RNA 6000 Nano Kits (Agilent – 5067-1511) 500 ng total RNA per sample was used to prepare libraries using Illumina's TruSeq RNA Library kit, according to manufacturer's protocol.

Runs: 1 run, 19.8M spots, 2G bases, 631.8Mb

Run	# of Spots	# of Bases	Size	Published
SRR28038204	19,819,907	2G	631.8Mb	2024-02-26

ID:: 31974031

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