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SRX22919677: GSM7978846: CD3; Drosophila melanogaster; RNA-Seq
1 ILLUMINA (Illumina NovaSeq 6000) run: 20.9M spots, 6.3G bases, 1.9Gb downloads

External Id: GSM7978846_r1
Submitted by: igred
Study: Characterisation of TET function in drosophila larval central nervous system
show Abstracthide Abstract
Enzymes of the Ten Eleven Translocation (TET) family play a key role in the regulation of gene expression in many species by oxidizing 5-methylcytosine (5mC), a prominent epigenetic mark, into 5-hydroxymethylcytosine (5hmC). Yet, TET proteins also have non-canonical modes of action beyond 5mC oxidation, notably in Drosophila, whose genomes is devoid of 5mC. Here, we used a combination of NGS analyses to studied the funciton and mode of action of Tet in the central nervous system of Drosophila larvae. Overall design: All experiments were performed with biological replicates (duplicates for ChIP-seq and CUT&RUN; quadruplicates for RNA-seq). w1118 (wt)were used as control lines.
Sample: CD3
SAMN38881045 • SRS19887641 • All experiments • All runs
Library:
Name: GSM7978846
Instrument: Illumina NovaSeq 6000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: The central nervous systems from wild type (w11118), Tet null or Tet CD (catalytic dead) mutant third instar Drosophila melanogaster larvae were dissected in PBS. For RNA-seq, total RNA was extracted with Trizol. ChIP-seq were performed as described in Loubiere et al. (Bio Protocol 2017). CUT&RUN were performed as described in Ahmad et al. (PLoS Genet. 2019). For RNA-seq, libraries were prepared using the Illumina TruSeq RNA Library Prep kit. For ChIP-seq and CUT&RUN, libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina.
Runs: 1 run, 20.9M spots, 6.3G bases, 1.9Gb
Run# of Spots# of BasesSizePublished
SRR2724141620,927,2526.3G1.9Gb2024-04-02

ID:
30984564

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    The wearing away of a tooth as a result of tooth-to-tooth contact, as in mastication, occurring only on the occlusal, incisal, and proximal surfaces. It is chiefly associated ...<br/>Year introduced: 1997
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