GSM7952016: Run2-MC004_L; Rattus norvegicus; RNA-Seq - SRA

SRX22818100: GSM7952016: Run2-MC004_L; Rattus norvegicus; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 11.2M spots, 1.6G bases, 666.3Mb downloads

External Id: GSM7952016_r1

Submitted by: 251 Sir Frederick Banting Driveway, Health Canada

Study: Comparative genomic analysis of Fischer F344 rat livers exposed for 90 days to 3-methylfuran or its parental compound furan.

PRJNA1049840 • SRP476481 • All experiments • All runs

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Transcriptomic data obtained by RNA-seq from male Fischer 344 rats treated with furan and 3-methylfuran were compared and contrasted. Microarray data from the same rats treated with furan was also used to compare microarray to RNA seq analysis. Overall design: Compare trascriptomics data between furan and its derivative 3-methylfuran in male Fischer 344 rats using RNA-seq while at the same time comparing microarray data from the same rats treated with furan to that of RNA-seq data from both furan and 3-methylfuran.

Sample: Run2-MC004_L

SAMN38716895 • SRS19799650 • All experiments • All runs

Organism: Rattus norvegicus

Library:

Name: GSM7952016

Instrument: NextSeq 500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Total RNA was extracted from flash frozen tissue samples using Qiagen miRNeasy kits. 20mg of frozen liver was placed in pre-filled tube containing Qiazol reagent and Zirconium beads, then homogenized 2 x for 20 seconds. Subsequent steps were followed as per manufacture's instructions. 3-methylfuran cDNA libraries were constructed using Illumina TruSeq Stranded mRNA protocol. Furan cDNA libraries were prepared with the Illumina Stranded mRNA prep kit (updated release).

Runs: 1 run, 11.2M spots, 1.6G bases, 666.3Mb

Run	# of Spots	# of Bases	Size	Published
SRR27136222	11,232,748	1.6G	666.3Mb	2024-01-08

ID:: 30860045

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