Instrument: NextSeq 500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: RNA in the heart apex was isolated using Trizol and measured (NanoDrop spectrophotometer ND-1000, ThermoFischer Scientific Inc.). Polyadenylated mRNA fraction was isolated using Poly(A) Beads (NEXTflex). Sequencing libraries were prepared using the Rapid Directional RNA-Seq Kit (NEXTflex)