GSM2344888: VEGF Trap_Treated_3; Mus musculus; RNA-Seq - SRA

SRX2245374: GSM2344888: VEGF Trap_Treated_3; Mus musculus; RNA-Seq
1 ILLUMINA (NextSeq 500) run: 12M spots, 911.1M bases, 364.1Mb downloads

Submitted by: NCBI (GEO)

Study: Therapeutic effects of Tie2 activation in treatment of neovascular age-related macular degeneration over conventional VEGF blockade.

PRJNA348410 • SRP091537 • All experiments • All runs

show Abstracthide Abstract

To investigate a potential therapeutic effect of ABTAA on NV-AMD, we employed a mouse model of laser-induced CNV, that mimics human NV-AMD. A laser photocoagulation on mouse retinas was performed to rupture RPE and Bruch's membrane, and then the effects of ABTAA were compared with those of VEGF-Trap. Overall design: RPE-choroidal complex was obtained from enucleated eyes and flat-mounted on polyethylene naphthalate membrane (Carl Zeiss). CNV and surrounding tissues (a two-fold larger diameter than CNV itself), including RPE and choroid, were collected using laser capture microdissection (PALM MicroBeam, Carl Zeiss) and subjected to RNA isolation.

Sample: VEGF Trap_Treated_3

SAMN05908297 • SRS1745922 • All experiments • All runs

Organism: Mus musculus

Library:

Instrument: NextSeq 500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: collected using laser capture microdissection (PALM MicroBeam, Carl Zeiss) RNA was harvested using Trizol reagent (Invitrogen). 500 ng of total RNA was used for the construction of sequencing libraries. RNA libraries were prepared for sequencing using LEXOGEN Quant-Seq Library Prep Kit (Cat#001.24) standard protocols.

Experiment attributes:

GEO Accession: GSM2344888

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 12M spots, 911.1M bases, 364.1Mb

Run	# of Spots	# of Bases	Size	Published
SRR4423190	11,987,644	911.1M	364.1Mb	2018-10-01

ID:: 3294920

SRA

Sequence Read Archive

Result Filters

Send to:

Supplemental Content

Related information

Search details

Recent activity