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SRX22385090: GSM7883717: Pruned.2; Pisum sativum; RNA-Seq
1 ILLUMINA (Illumina NovaSeq 6000) run: 22.5M spots, 6.7G bases, 2.1Gb downloads

External Id: GSM7883717_r1
Submitted by: Instituto de Biología Molecular y Celular de Plantas, IBMCP (UPV-CSIC)
Study: Identification of factors that modulate Proliferative Arrest under the influence of seeds
show Abstracthide Abstract
In monocarpic plants, after the production of a certain number of fruits/seeds, the activity of the Shoot Apical Meristem (SAM) and of the meristems of the secondary axes stop in a coordinated way, leading to the cessation of flower production. This phenomenon has been called Proliferative Arrest (PA). It can be assumed as a general phenomenon in monocarpic plants, as it has been described in different species of this economically important group of plants. Studies in the model species Arabidopsis thaliana and Pisum sativum have allowed the identification of factors that affect PA timing. One of them is the production of fruits and seeds. With this RNA-seq, we have identified factors that contribute to the modulation of PA in a seed-dependent manner in Pisum sativum. Overall design: To better understand the influence of seeds in modulating Proliferative Arrest, samples of Shoot Apical Meristem were collected at four different stages of plant development (with/withouth seeds; active/arrested meristems)
Sample: Pruned.2
SAMN38122688 • SRS19431273 • All experiments • All runs
Organism: Pisum sativum
Library:
Name: GSM7883717
Instrument: Illumina NovaSeq 6000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: RNA from Shoot Apical Meristem samples at the different stages described was extracted using the RNeasy Mini Kit (Qiagen) and treated with DNaseI (Turbo DNA-free kit INVITROGEN; Ref-AM1907) following the manufacturer's instructions. The quality of the RNA was checked on an Agilent 2100 Bioanalyzer instrument using the RNA6000 nano kit. Eukaryotic Transcriptome Library/Strand specific libray (mRNA-seq poly A enrichment) was constructed using Illumina NovaSeq 6000
Runs: 1 run, 22.5M spots, 6.7G bases, 2.1Gb
Run# of Spots# of BasesSizePublished
SRR2668519222,529,1826.7G2.1Gb2024-06-05

ID:
30366921

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