Name: GSM7848971
Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: A sample consisted of 15 pooled tadpoles from each treatment and each was repeated 3 times. Tissue was extracted using TRIzol (Thermo Fisher Scientific) as per the manufacturer's protocol, and total RNA quality and quantity was assessed using a NanoDrop spectrophotometer (Thermo Fisher Scientific). RNA library preparation was done with the Illumina Stranded Total RNA with Ribo-Zero Plus. Libraries were then multiplexed, and an rRNA depletion run using single-end, 75-nucleotide sequencing was performed on Illumina HiSeq 2500.