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SRX22145127: GSM7848971: control, large group, stage 35, sample 3; Xenopus laevis; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 16M spots, 1.2G bases, 502.7Mb downloads

External Id: GSM7848971_r1
Submitted by: Wyss Institute at Harvard University
Study: Embryos Assist Each Other's Morphogenesis: calcium and ATP signaling mechanisms in collective resistance to teratogens (Stage 35)
show Abstracthide Abstract
We investigated whether normal embryonic developmental cues are only at the level of cells within the embryo or whether they exist in interactions between embryos. To explore this, we subjected groups of embryos with disruptors of normal development while varying group size. RNA-seq analysis revealed a small set of transcripts specific for teratogen response in large groups but not small ones. Overall design: Comparative gene expression profiling analysis of RNA-seq data for metamorphosing X. laevis tadpoles exposed to thioridazine. Control and exposed treatments in triplicate.
Sample: control, large group, stage 35, sample 3
SAMN37883706 • SRS19204848 • All experiments • All runs
Organism: Xenopus laevis
Library:
Name: GSM7848971
Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: A sample consisted of 15 pooled tadpoles from each treatment and each was repeated 3 times. Tissue was extracted using TRIzol (Thermo Fisher Scientific) as per the manufacturer's protocol, and total RNA quality and quantity was assessed using a NanoDrop spectrophotometer (Thermo Fisher Scientific). RNA library preparation was done with the Illumina Stranded Total RNA with Ribo-Zero Plus. Libraries were then multiplexed, and an rRNA depletion run using single-end, 75-nucleotide sequencing was performed on Illumina HiSeq 2500.
Runs: 1 run, 16M spots, 1.2G bases, 502.7Mb
Run# of Spots# of BasesSizePublished
SRR2644048816,001,5891.2G502.7Mb2023-12-02

ID:
30097632

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