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SRX22106492: GSM7844251: W3H150m_rep5_MGF; Solanum lycopersicum; RNA-Seq
1 ILLUMINA (HiSeq X Ten) run: 12.5M spots, 1.9G bases, 786.2Mb downloads

External Id: GSM7844251_r1
Submitted by: Plant Genomic Network Research Team, CSRS, RIKEN
Study: Determination of transcriptomic changes associated with heat stress in ethanol treated vs non-treated control tomato plants II
show Abstracthide Abstract
We report the gene expression profiles of tomato seedlings and fruits treated with or without ethanol under heat stress conditions. Overall design: Seeds of a tomato (Solanum lycopersicum L.) cultivar, “Micro-Tom” were grown in pots containing horticultural cray granule soil at 22 °C with a 16 h light (110 to 140 µmol m-2 s-1 photon flux density) / 8 h dark cycle. Sixteen-day-old plants grown in pots were used for ethanol pretreatment. The bottoms of the pots were dipped into 20 mM ethanol solution and kept for 3 days. After ethanol pretreatment, the pots were placed in an air incubator set at 50 °C and maintained for the indicated time periods as heat stress treatment. During the heat stress treatment, the pots were electrically rotated in the air incubator to avoid the local effect. After the heat stress treatment, the pots were returned to the growth room and maintained for the indicated time periods.
Sample: W3H150m_rep5_MGF
SAMN37849473 • SRS19171383 • All experiments • All runs
Library:
Name: GSM7844251
Instrument: HiSeq X Ten
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Total RNAs were extracted using purelink Plant RNA Reagent (Thermo Fisher Scientifc Inc., Waltham, MA, USA). The sequencing library was prepared using the Lasy-Seq method (Kamitani et al. 2019). Specifically, 200 ng of total RNA was used per sample.
Runs: 1 run, 12.5M spots, 1.9G bases, 786.2Mb
Run# of Spots# of BasesSizePublished
SRR2640074312,461,6751.9G786.2Mb2024-03-13

ID:
30038354

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