GSM7767090: T312 [HA_012]; Canis lupus familiaris; RNA-Seq - SRA

SRX21666912: GSM7767090: T312 [HA_012]; Canis lupus familiaris; RNA-Seq
1 ILLUMINA (NextSeq 2000) run: 13.8M spots, 1.4G bases, 440.3Mb downloads

External Id: GSM7767090_r1

Submitted by: Biomedical Data Science, Dartmouth College

Study: Neoadjuvant intratumoral immunotherapy with cowpea mosaic virus induces local and systemic antitumor efficacy in canine mammary cancer patients.

PRJNA1014121 • SRP459174 • All experiments • All runs

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This study evaluated the efficacy, safety, and effects on treated and untreated tumors, and clinical impact of neoadjuvant intratumoral empty cowpea mosaic virus (eCPMV) immunotherapy in CMC patients. Overall design: Impact of neoadjuvant intratumoral empty cowpea mosaic virus immunotherapy on canine mammary cancer gene expression profiles generated by RNA-seq and tumor size in treated and untreated patients.

Sample: T312 [HA_012]

SAMN37319529 • SRS18833463 • All experiments • All runs

Organism: Canis lupus familiaris

Library:

Name: GSM7767090

Instrument: NextSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Snap-frozen tumor samples were used for rna isolation. RNA was extracted using the Rneasy Mini Kit (Qiagen, Redwood City, CA) after homogenization by beating with 2mm stainless steel beads in Qiagen RLT buffer. RNA-seq libraries were prepared using the Lexogen QuantSeq Rev kit following manufacturer's protocols and sequencing as single-end, 100bp reads on a NextSeq2000 instrument.

Runs: 1 run, 13.8M spots, 1.4G bases, 440.3Mb

Run	# of Spots	# of Bases	Size	Published
SRR25948297	13,805,632	1.4G	440.3Mb	2023-09-12

ID:: 29330936

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