Name: GSM7713999
Instrument: Illumina HiSeq 4000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: Right and left ventricular heart specimens were dissected from total hearts isolated from euthanized, male Tg-BBLN mice and non-transgenic FVB/N mice (age: 3-4 months). Dissected heart specimens were rapidly frozen in liquid nitrogen. For RNA isolation, right and left heart specimens were pulverized under liquid nitrogen, and total RNA was isolated with the RNeasy midi kit (Qiagen). RNA libraries were prepared for transcriptome sequencing by GATC Biotech AG, an Eurofins Genomics Company, using standard Illumina protocols in frame of the INVIEW Transcriptome Discover protocol. For library preparation, mRNA was fragmented, and random hexamer-primed cDNA synthesis was performed. Illumina paired-end read sequencing (2 x 150 bp) with guaranteed 30 million reads per sample was performed by the Genome Sequencer Illumina HiSeq, in the sequence mode HiSeq 4000.