U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

SRX21113835: GSM7655438: Synechococcus cells, labA-KO, replicate 3; Synechococcus elongatus PCC 7942 = FACHB-805; RNA-Seq
1 ILLUMINA (Illumina NovaSeq 6000) run: 28M spots, 8.5G bases, 2.7Gb downloads

External Id: GSM7655438_r1
Submitted by: Biological Sciences, Vanderbilt University
Study: Clocking out and letting go to unleash green biotech applications in a photosynthetic host
show Abstracthide Abstract
Cyanobacteria are attractive hosts for producing pharmaceuticals, renewable fuels, and chemicals due to their ability to use sunlight as their energy source. Despite the application of traditional genetic tools such as the identification of strong promoters to enhance the expression of heterologous genes, however, cyanobacteria have lagged behind other microorganisms such as E.coli and yeast as economically efficient bioreactors. The previous approaches have ignored large-scale constraints within cyanobacterial metabolic networks on transcription, predominantly the pervasive control of gene expression by the circadian (daily) clock. Here we adopt a novel strategy and show that reprogramming gene expression within cyanobacteria by inactivation of the circadian oscillator coupled with release of circadian repressor elements in the transcriptional regulatory pathways enables a dramatic enhancement of expression in cyanobacteria of heterologous genes encoding both catalytically active enzymes and polypeptides of biomedical significance. Overall design: To investigate the potential of the genes labA and kaiA in enhancing production of bioproducts, we created strains of the cyanobacterial model Synechococus elongatus PCC 7942 in which i) labA had been knocked-out, ii) kaiA had been overexpressed through a Zinc-induced promoter or iii) both modifications were done, and perfomed RNAseq on cultures that were given a 12h dark pulse and were collected after 12h in LL.
Sample: Synechococcus cells, labA-KO, replicate 3
SAMN36660212 • SRS18382429 • All experiments • All runs
Library:
Name: GSM7655438
Instrument: Illumina NovaSeq 6000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: RNA was extracted from cells preserved in RNA Protect (Qiagen) and processed with the Rneasy Mini Kit (Qiagen). DNA contamination was removed with Dnase I (NEB). Library preparation and rRNA depletion was performed using the NEBNext® rRNA Depletion Kit for Bacteria (New England BioLabs).
Runs: 1 run, 28M spots, 8.5G bases, 2.7Gb
Run# of Spots# of BasesSizePublished
SRR2537479827,997,8308.5G2.7Gb2024-04-09

ID:
28548744

Supplemental Content

Search details

See more...

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...