GSM7610930: 2C Early S Expt. 1 - Sample B3; Mus musculus; OTHER - SRA

SRX21024416: GSM7610930: 2C Early S Expt. 1 - Sample B3; Mus musculus; OTHER
4 ILLUMINA (NextSeq 500) runs: 8.6M spots, 1.2G bases, 553.3Mb downloads

External Id: GSM7610930_r1

Submitted by: The University of Copenhagen

Study: The establishment of a DNA replication timing program in the early embryo.

PRJNA994886 • SRP449564 • All experiments • All runs

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Single Embryo Repli-Seq was utilized to investigate the replication timing in zygotes and 2-cell cleavage stage embryos. The aim was to understand the temporal progression of DNA replication during the early stages of embryonic development in mammals. Overall design: The replication timing of zygotes and 2-cell stage embryos was analyzed using Single Cell RepliSeq.

Sample: 2C Early S Expt. 1 - Sample B3

SAMN36451878 • SRS18297213 • All experiments • All runs

Organism: Mus musculus

Library:

Name: GSM7610930

Instrument: NextSeq 500

Strategy: OTHER

Source: GENOMIC

Selection: other

Layout: SINGLE

Construction protocol: DNA extraction for single-cell RepliSeq was conducted following established protocols as described in a previous publication (PMID: 33230331). Library construction was carried out using the protocols established by Miura et al., 2020 (PMID: 33230331), incorporating IDT for Illumina unique dual indexes and the KAPA Hyper Prep Kit from Roche. single cell RepliSeq

Runs: 4 runs, 8.6M spots, 1.2G bases, 553.3Mb

Run	# of Spots	# of Bases	Size	Published
SRR25279385	2,160,796	313.1M	138.4Mb	2024-05-23
SRR25279386	2,120,668	307.5M	136.8Mb	2024-05-23
SRR25279387	2,168,259	314.3M	140.5Mb	2024-05-23
SRR25279388	2,138,167	309.8M	137.6Mb	2024-05-23

ID:: 28458876

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