Name: GSM7585195
Instrument: Illumina NovaSeq 6000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: RNA was isolated from mouse tissues using the Ambion RNAqueous Total RNA Isolation Kit (Invitrogen). ~25 mg of solid tissue was used as starting material. Concentration of RNA samples was determined using the Qubit RNA HS assay kit (Invitrogen). Total RNA isolated as described above was checked for quality using an Agilent 2100 Bioanalyzer. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection.